Nanoliposomes of Marine Lecithin, a New Way to Deliver TGF B1
DOSTERT ; KAHN ; MENU ; MESURE ; CLEYMAND ; LINDER ; VELOT ; ARAB-TEHRANY
Type de document
ARTICLE A COMITE DE LECTURE REPERTORIE DANS BDI (ACL)
Langue
anglais
Auteur
DOSTERT ; KAHN ; MENU ; MESURE ; CLEYMAND ; LINDER ; VELOT ; ARAB-TEHRANY
Résumé / Abstract
Studies made these last decades showed how TGF B family molecules have a crucial role in cell signalling in order to stimulate the differentiation of stem cell into desired phenotype in time and dose dependence, making them essential in regenerative medicine. Therefore, controlling TGF-²s cell administration during culture or healing is essential. Thus, we focused on a nanoliposome as natural nanocarrier for drug delivery system used since few years in pharmaceutics and cosmetics applications. This work studied at first culture effects on human mesenchymal stem cells (hMSCs) harvested from Wharton's Jelly human umbilical cord of TGF-²1 encapsulated or not into salmon lecithin nanoliposomes from 0 to 2.5 ng/mL up to 14 days. During culture, cell metabolic activity, proliferation and shape were investigated. In parallel, salmon lecithin and nanoliposomes were analysed according to routine physico-chemical analyses such as lipid composition, nanoliposome size and morphology. These analyses showed that salmon lecithin was composed of a high level of polyunsaturated fatty acids essentially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Moreover, neither nanoliposome nor TGF-²1 alone or encapsulated into nanoliposome showed any cytotoxicity according to the concentration studied herein. Finally, nanoliposome solutions have a higher impact on cell proliferation than TGF-²1 and compared to TGF-²1 alone, these solutions limit maximum cell number and maintain a higher level cell metabolic activity. In conclusion, this study represents a first experimental approach based on morphological cell and cytocompatibility which showed no in vitro negative effect on hMSCs.
Source
Journal of Biomaterials and Tissue Engineering, num. 11, pp.1163-1170 p.
Editeur
American Scientific Publishers
